Multiplexed fluorescence ELISA assays on mirrorball®
Enzyme-linked immunosorbant assays (ELISA) can be used in multiple stages of the drug discovery process to help identify and measure the cellular responses to therapeutic or toxic molecules. The most common ELISA format is the sandwich assay. Briefly, a capture antibody is immobilised onto a solid support surface, the analyte to be quantified (eg, cytokine, peptide, protein) binds to the capture antibody and can then be detected by a biotinylated secondary antibody. Incubation with streptavidin-conjugated HRP (horse radish peroxidise), followed by a colorimetric substrate facilitates assay quantitation.
Download the whitepaper now to find out more about:
- Enzyme-linked immunosorbant assays (ELISA)
- Fluorescence ELISA process
- The mirrorball fluorescence cytometer
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